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GreenLight™ 960: Same-Day Analysis Of Aerobic Plate Counts (APCs,TVCs) In Raw Meat Samples

Source: MOCON, Inc.
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White Paper: GreenLight™ 960: Same-Day Analysis Of Aerobic Plate Counts (APCs,TVCs) In Raw Meat Samples

By MOCON, Inc.

An increasing appreciation for the importance of food and microbiological safety has resulted in a demand for a more rapid, high-throughput method for total viable count (TVC) quantification to deal with the increasing numbers of samples that require testing. The industry standard for TVC determination (ISO.4833.2003), also known as aerobic plate count, is widely used but presents users with some very significant drawbacks. The method is both materials and labour intensive, requiring the preparation and analysis of multiple agar plates per sample. More importantly, the method is slow, with 48-72 hours typically required for a definitive result.

Luxcel Biosciences has addressed these limitations with the development of the GreenLight™ 960 ; a microtitre-plate based assay which provides a rapid high-throughput method for the assessment of aerobic bacterial load through analysis of microbial oxygen consumption. When applied to the determination of Total Aerobic Viable Counts in raw meat (Beef samples) results are generated in 1-12 hours depending on microbial load.

The GreenLight™ assay is a simple ‘mix and measure' assay: Using food homogenates, prepared as for traditional TVC testing (ISO:4833:2003), probe, sample & oil are added in sequence and resultant aerobic TVC values quantified using an appropriate calibration.

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White Paper: GreenLight™ 960: Same-Day Analysis Of Aerobic Plate Counts (APCs,TVCs) In Raw Meat Samples

MOCON, Inc.

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